I.  REVIEW OF THE GENERAL PROCEDURE FOR THE ENRICHMENT, ISOLATION AND IDENTIFICATION OF BACTERIA as shown on the main enrichment/isolation page:

ENRICHMENT AND ISOLATION						PURE CULTURE WORK
SOURCE MATERIAL		BROTH ENRICHMENT		PLATING FOR ISOLATION		STOCK CULTURES		CHARACTERIZATION & IDENTIFICATION
•Consider inoculum: what organisms may or may not be present. •May pre-treat inoculum, e.g., by heat-shocking.		•Usually is selective. •May be skipped altogether.		•Usually selective or selective-differential – but not always!!
		Throughout procedure, appropriate media and incubation conditions must be considered.

II.  A "WORKSHEET" TO HELP US ORGANIZE AND SUMMARIZE THE SPECIFIC FACTORS that allow isolation of the various types of microorganisms in these experiments:

	10.2 Streptomyces	11.1 Purple "non-sulfur" photosynthetic bacteria	11.2 Bacillus	11.3 Free-living N2-fixing bacteria
Special properties of desired organism that can be exploited to help us "sort it out" from others.
Likely source material (habitat of organism or where the organism is a significant contaminant).
Treat sample to enhance isolation?
Need to use enrichment?
Factors to consider for the enrichment & plating media:* •Selective by restriction of nutrients or addition of selective agents? •Special sources of C, N, energy?	(Why is 2nd plating medium an all-purpose medium?)	**
Special incubation conditions?		**
Detectable features of the desired organism that help us in picking and identifying it.		**
Any special, additional tests on the isolates? (That is, tests not essential to identification to the genus level.)

  *  For the desired type of organism, the medium/media must include the required elements (including trace elements) and whatever growth factors are needed. Note that these organisms are all heterotrophs and organotrophs. (A review of Appendix D in the manual is useful; it is also reproduced here.)

**  See question 11B on page 162 in the manual. How would we want to grow the purple non-sulfur photosynthetic bacteria to maximize their detection and minimize growth of other organisms – as phototrophs or as chemotrophs?