As of January 2007, the format of the course was changed such that many of the in-lab experiments became on-line exercises where it was found appropriate to do so. Consequently, this course website was "retired" and present-day UW-Madison Microbiology 102 students go to the "Learn@UW" website for course announcements and links to resources (and other useful things including grade summaries).
E. B. Fred
E. B. Fred Hall (Click on image for
16 pages of demolition photos.)
MAJOR COURSE INFORMATION:
LINKS TO MORE GOOD STUFF including the following
Introduction to Bacteriology 102
This course is required by a number of majors including Medical Technology, Nutrition, Dietetics, the Physician's Assistant Program and Nursing. If you intend to major in Bacteriology, we have Bact. 304 (and the corresponding lecture course, Bact. 303) for which organic chemistry is a prerequisite. Bact. 102 does not have to be taken the same semester as Bact. 101, but please do not sign up for 102 unless you are taking – or have taken – Bact. 101 or an equivalent course.
Bacteriology 102 has a lot of substance. It is not a survey course that just celebrates the shapes and colors of the bacterial world. We try to amplify and add to the material given in the lecture course. Likewise, the lecture course serves as a supplement to this course. From time to time, students have been known to "graduate" from this course to a lab technician position out in the "real world." So there are a number of basic concepts which Bact. 102 students and aspiring microbiologists must learn and re-use throughout and possibly beyond this semester.
The following items form a major part of the course, and a good understanding of them will be expected when you begin a course for which Bact. 102 is a prerequisite – such as our food microbiology lab course (Bact./Food Science 324).
There are really not that many basic principles to understand. However, they work together and build over the course of the semester. You can wind up not only knowing lots of stuff but also how to come up with logical ways of accomplishing things. We do not indulge in the memorization of lots of trivia – i.e., things that can fill up tables in reference books. From time to time we may throw out a "thought question" to test your understanding of basic microbiology, and no mass memorization of minutiae will help in those situations.
Keeping up with the material is necessary, and this also applies to the lecture course. Do not leave things until cramming becomes a necessity. Come to lab prepared! You can always consult the lab schedule to see what is coming up and then plan accordingly. Making a "flow chart" ahead of time can help one sail through the lab period with less problem.
Keeping a notebook is also necessary. You will need to take good notes of the lab lectures including the material presented on the blackboard. You may find there is no space in the manual to record certain observations, and you will want to make note of certain material which may need your attention before the next lab. You can collect your "flow charts" in your notebook as well. A loose-leaf notebook which can be arranged chronologically or according to experiment number is a suggestion. Such a notebook can also be handy for keeping all of your handouts.
If you find you have questions about the material as you come to lab, you and the class can benefit greatly if you bring up these questions during the opening lab lecture. Before lab, you may find one or more of the instructors struggling to get things ready for the lab section, and they are sometimes unavailable until the period begins.
Besides the various elements of your lab experience which include note-taking and the use of the manual and handouts – we have our website to help with a good review. A few things may be explained more clearly on the web than in the manual (such as growth curves, dilution theory, oxygen relationships and differential media), and you will find some of the lab demonstrations reproduced in blazing color. This is also the place to find answer keys for certain questions and problems in the manual.
This is a fairly easy-going lab and also has been quite safe as long as you remember your "aseptic technique"! Make sure your insurance situation is in order. We do not have a blanket insurance policy for students taking lab courses, but the lab instructor can be held fully accountable for negligence, if that ever happens.
Study Hints and Answer Keys
As mentioned above and also on the first day of lab, be sure to keep up with the material throughout the semester! You might encounter a lot of "fine print" in the manual and also in the resource material on our websites – i.e., the Bacteriology 102 Website (where you are now) and also our General Microbiology Pages (which are indexed here). To bring some focus to this material, the Update Page (example here) not only (1) reiterates the required assignments announced in lab (for reading and for handing in) but also (2) links to recommended supplementary material on the web (our websites and those of others).
Our general microbiology pages have been found to be helpful by others who use the web. For example, do a search with any good search engine for "catabolism" or "dilution plating" and see how our page ranks.
Quite often the web material explains things better than what is in the manual. For example, you may find that the first page of our Dilution Theory site goes well with the plating procedure in Experiment 1, and the second page of this site fits in well with the hamburger plating in Exp. 4. Both of these pages explain "dilution theory" more clearly and carefully than what is in the manual (in Appendix C). Many of the handouts given out over the course of the semester have their origins in our web pages. One could conceivably get along without using the web this semester, but the keys to the questions and problems in the lab manual will only be found on our website. (Links to the keys are below.)
So, as you search around the web, let us know if you come upon some particularly useful material, and we will add it to our growing list of links.
As you go through the procedures – whether you are reviewing for a quiz or otherwise – ask yourself "what did I do" and "why?" Also: "What did I get out of my results?" A good set of notes from the lab lectures is especially valuable. Keeping up with the lecture course also "greases the wheels" and much of what you learn in 102 can help with 101!
The list of lab manual topics found here (bottom half of page – with links) may be of some help as a study guide.
Links to the keys showing the answers and solutions to the sample questions and problems in the manual are listed below. Remember that what we have in mind regarding these keys is that you should go through the sample questions and problems first on your own (i.e., quiz yourself!), and then check your results with the keys. For true/false questions, be sure you know why a given statement may be true(+) or false(O).
Following is a list of some special sets of questions on our website which are good to think about, as you may encounter similar situations someday. It is probably best to go over these questions after the mid-semester point when they will make more sense; there is no need to worry about them for the first quiz! Presently we don't have keys to these questions on the web.
Point System and Grading Scale
|Exp. 2 Unknown (gram stain)||5||Quiz no. 1*||40|
|Exp. 6 Unknown (motility)||5||Quiz no. 2*||40|
|Exp. 7.2 Unknown (general/mixed)||10||Quiz no. 3*||40|
|Exp. 14.1 Unknown (enteric/mixed)||5||Formal Report||50|
|Exp. 17 Dichotomous Key||10||Growth Curves and Calculations||10|
|Exp. 17 Unknown (general/mixed)||15||Water Analysis||5|
|Problem Set no. 1||10||Miscellaneous Points||5|
|Problem Set no. 2||10||Final, Cumulative Exam*||100|
TOTAL POINTS = 360
* For the usual Summer Session, there are two quizzes at 50 points each,
93-100% = A
About the quizzes:
Use the grading scale on the right as a guide. The final letter grade will not be based on a stricter scale. At any time during the semester, you can easily calculate your current percentage by dividing your accumulated points by the number of points taken by that time (and then multiplying by 100%).
A word of caution: Do not base how much you study for any quiz or exam on what your current percentage is or might become. One can never tell how "hard" or "easy" any particular quiz or exam may be. It will be to your advantage to follow the advisory given above in the section on "Study Hints and Answer Keys."
There will be no problem taking the final exam at a different time if you find you have other finals piling up together.
Click on image for the classic Linda Barry cartoon about learning microbiology in school.
There are lots of educational resources available on the Web which one can access, and one can always do a search. Some are listed here – not in any particular order:
The content of this Bacteriology 102 Site has copyright by John Lindquist and found its permanent sanctuary on www.splammo.net in 2001.